Preparation of antigens from different sources including small organic molecules, peptides, oligonucleotides, recombinant proteins, glycoproteins, polysaccharides and cells.
Monoclonal, Polyclonal.
Hybridoma monoclonal and recombinant monoclonal production up to 1g scale.
Antibody purification by affinity chromatography (protein A, G, L, CaptureSelect), antigen affinity chromatography (including enrichment/ depletion strategies) and size exclusion chromatography.
Antibody characterisation by antigen ELISA, immunoblotting, affinity ranking, antibody pair identification, isotyping, SDS-PAGE, IEF and endotoxin assay.
Conjugation of haptens to carrier proteins to prepare immunogens.
Conjugation of antibodies and antibody fragments to enzymes, fluorophores, biotin, particles and surfaces for use in various antibody-based methods including EIA, LF, IHC, ICC, IF, IP.
Enzyme immunoassay, immunoblot and lateral flow assays developed using generated or sourced reagents. Fit for purpose qualification/ validation work carried out.
Hybridoma culture, hybridoma stabilisation, serum-free culture.
Recombinant antibody and protein production in mammalian cells.
Lymphocyte preparation from peripheral blood and tissue.
Cell banking and two-site safe cell storage.
Mycoplasma testing, hybridoma sequencing, reagent lyophilisation.